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. 2017 Sep 6;40(5):1435–1443. doi: 10.3892/ijmm.2017.3120

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Copyright: © Guo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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(A) Western blot detection of PI3K expression levels in cells following transfection with miR-7-5p mimics, TFF3 plasmid and LY294002. GAPDH served as the loading control. (B) Quantitative analysis of PI3K protein expression levels normalized to GAPDH levels. The assays were performed in triplicate. All values are presented as the means ± standard deviation of three replicates. ^*P<0.05. PI3K, phosphoinositide 3-kinase; TFF3, trefoil factor 3; miR, microRNA; GAPDH, glyceraldehydes-3-phosphate dehydrogenase; NC, negative control.