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. 2017 Sep 6;40(5):1435–1443. doi: 10.3892/ijmm.2017.3120

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Copyright: © Guo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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(A) Western blot detection of Akt and p-Akt levels in cells following transfection with miR-7-5p mimics, TFF3 plasmid and LY294002. GAPDH served as the loading control. (B) Quantitative analysis of Akt and p-Akt protein expression levels normalized to GAPDH levels. The assays were performed in triplicate. All values are presented as the means ± standard deviation of three replicates. ^*P<0.05. p, phosphorylated; TFF3, trefoil factor 3; miR, microRNA; GAPDH, glyceraldehydes-3-phosphate dehydrogenase; NC, negative control.