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. 2017 Sep 7;40(5):1444–1456. doi: 10.3892/ijmm.2017.3127

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Copyright: © Ren et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of ferulic acid (FA) on thye ischemia-induced apoptosis of PC-12 cells. (A) Annexin V/PI staining was used to determine apoptosis by flow cytometry. (B) Histograms showing the ratio of dead cells to the total nuclei. (C) Nuclearmorphology was determined by Hoechst 33258 staining; the white arrows indicate the apoptotic cells, at ×400 magnification. (D) Histograms showing the ratio of condensed nuclei to total nuclei. (E and F) The expression of apoptosis related proteins was examined by qPCR and western blot analysis.