Fig 3. Cold storage plus transplantation impairs renal function and mitochondrial morphology.
(A) Serum creatinine and blood urea nitrogen (BUN) from the sham and 18 hr CS/Tx rats were analyzed using hand-held clinical chemistry analyzer (iSTATTM) and Chem8+ cassettes as described in materials and methods. Values were expressed as Mean ± S.E.M. (n = 4). The unpaired Student’s t test was used to compare the means between sham and CS/Tx; * indicates means are significantly different from sham (P < 0.05). (B) Electron micrographs revealed normal mitochondria in sham kidneys (cortical region), but 18 hr CS/Tx kidneys show rounded, fragmented mitochondria with dense aggregates. Images are representative of n = 3 in each group; bar, 500 nm. TEM was performed at the Georgia Reagents TEM Core Facility.