Fig 7. Renal cold storage alone or cold storage plus transplantation does not decrease all mitochondrial protein expression.

Renal extracts (30 ug) were resolved on SDS-PAGE gels and immunoblotted. Representative Core2 and MnSOD western blots showing distinct protein bands of Core2 (~49 kDa) and MnSOD (~ 24 kDa) in Sham, ATx, and CS/Tx (A) and comparing control to CS alone (B). Actin was used as a loading control. Densitometry evaluation of each blot (normalized to actin) is shown on the right panel. Values were expressed as Mean ± S.E.M. (n = 4). Unpaired Student’s t test and one-way ANOVA were used to compare the means.