Table 4. Weaker agreement between experimental results and model outcomes upon simulated constitutive activity (or external supply) of nodes in the absence of abscisic acid (ABA).
| Response category | Total number of cases | Cases that are consistent with experimental results | Cases that are inconsistent with experimental results | CPC range |
|---|---|---|---|---|
| Equivalent to wild type (signal-free) | 20 |
SCAB1 [83] |
Nitrite (1a) [56] |
0.0–0.002 |
| Close to wild type | 47 | AtRAC1 [82], SphK1/2 [107], PLDα [36], PP2CA [118], ROP11 [104], ABI1 [102], cGMP [57], ABI2 [102], H+ ATPase [133] | S1P/ PhytoS1P (1a) [70, 134], pHc increase (1a) [78], NO (1a) [56], cADPR (1a) [124], PA (1a) [114], 8-nitro-cGMP (1a) [57], InsP3 (1a) [135] | 0.0–0.0024 |
| Slightly increased response | 7 | TCTP [32], Microtubule Depolymerization [116] | Ca2+c (1a) [136] | 0.0028–0.03 |
| Significantly increased response | 5 | ROS [46, 137] | OST1 (2) [25], RCARs (2) [42, 130] | 5.8–25.2 |
The wild-type system in this case refers to an initial condition that represents open stomata in the absence of any closure signal, with initial node states summarized in S5 Table. Cases of constitutive activity of a source node that is ON in the initial condition can be considered as replicates of the wild-type simulation and are denoted “equivalent to wild type.” Node activations that lead to a CPC within the wild-type equivalent range (0–0.002) are denoted “close to wild type.” The “equivalent to wild type” and “close to wild type” categories lead to a final closure percentage of 0% and may include transient closure in a few simulations. “Slightly increased response” corresponds to a final closure percentage of 0%, with a higher degree of transient closure and a CPC larger than the highest wild-type equivalent CPC. The category “significantly increased response” indicates a nonzero final closure probability, including a 100% closure probability for RBOH or ROS constitutive activity. The nodes are in the order of increasing CPC in each category. Discrepancy type designations are as in Table 3 with the addition that the category “slightly increased response” is considered consistent with experimentally observed wild-type responses.
Abbreviations: CPC, cumulative percentage of closure. All other abbreviations are explained in S3 Table.