(A) SUPER IM combined signal for ESI of Agilent tuning mix ions obtained at different passes (1–81) under the following surfing conditions: TW speed was 103 m/s, TW amplitude was 50 V, TW sequence was 11110000, guard =15 V, rf amplitude of 340 Vpp at 822 kHz, interboard gap was 2.75 mm, 2.50 Torr pressure. (B) The signal intensity (i.e., peak area) calculated plotted against the number of passes from replicate measurements.