Table 1. List of the antibodies used in this study and the phosphoepitopes they recognize.
Phosphoepitope | Clone (Supplier) |
---|---|
pCD3ζ (Y142) | K25-407.69 (BD) |
pLck (Y505) | 4/LCK-Y505 (BD) |
pSyk (Y342) | I120-722 (BD) |
pSLP76 (Y128) | J141-668.36.58 (BD) |
pItk (Y180) | N35-86 (BD) |
pPLCg2 (Y759) | K86-689.37 (BD) |
pWIP (S478) | K32-824 (BD) |
p-p38 (T180/Y182) | 36/p38 (pT180/pY182) (BD) |
pERK1/2 (T203/Y205) | 20A (BD) |
p-c-Cbl (Y698) | 47/c-Cbl (BD) |
pJNK (T183/Y185) | N9-66 (BD) |
pNFkB p65 (S468) | #3039 (CST) |
pNFkB p65 (S529) | K10-895.12.50 (CST) |
pNFkB p65 (S536) | 93H1 (CST) |
pAkt (T308) | C31E5E (CST) |
pAkt (S473) | M89-61 (BD) |
pS6 (S235/236) | D57.2.2E (CST) |
p4EBP1 (T36/45) | 236B4 (CST) |
p-mTOR (S2448) | D9C2 (CST) |
p-mTOR (S2481) | #2974 (CST) |