Fig. 4.
Grip1/2 DKO mice show altered GluA2-Serine880 phosphorylation and GABAβ3 levels in brain tissues. 20 μg of tissue lysates from respective brain regions of WT and Grip1/2 DKO mice were immunoblotted using antibodies specific for the indicated proteins. Expression levels of these proteins were then normalized using tubulin. Note an increase in phosphorylated GluA2 at serine 880 (GluA2-pS880) in brain cortex (Panel a) and cerebellum (Panel b) and reduced levels of GABAβ3 in striatum (Panel c) of Grip1/2 DKO mice. N = 4–6 for each study cohort. Data presents as mean ± SEM. t-test was used to compare the mean between study cohorts; *, p < 0.05 **, p < 0.01.