Table 6.

Four types of wound imaging techniques

Imaging technique	Advantages	Limitations
Confocal laser scanning microscopy	Controllable depth of field Elimination of out of focus data Ability to collect optical sections—3D reconstruction Portable	Resolution limited by wavelength of light Photodamage from high intensity laser
Multiphoton microscopy	Deeper sectioning than confocal microscopy because infrared excitation results in less scattering Reduced photobleaching Optical section based on fluorescence	Photodamage at focal point Low efficiency from auto fluorescence excitation
Optical coherence tomography	Much larger field of view than CLSM or MPM Deeper tissue regions than MPM 3-D scattering-based section	Lower resolution than CLSM or MPM High computational demands
Hyperspectral imaging	Simple—analogous to taking a picture with a digital camera Quantification of wound healing—areas of different tissue types in healing wound Portable	Can only acquire reflectance data Region of interest selection subjective