Figure 6. CART123 are resistant to inhibition by M2-macrophages.
A. In a 5-day CFSE proliferation assay, CART123 are completely resistant to HL-polarized macrophage mediated inhibition. B. CART123 cells rapidly (day 1) recognize M2-macrophages, forming clusters around macrophages and clearing them by day 5 as shown by phase contrast microscopy (20X) and flow cytometry, respectively. C. CART19 but not CART123 cytokine secretion was significantly reduced in the presence of M2 (IL-4 polarized) macrophages in vitro.