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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2017 Jun 15;16(10):2191–2200. doi: 10.1158/1535-7163.MCT-16-0907

Figure 6. Double strand DNA breaks present within tumor tissue but absent within surrounding tissue showing enhanced vascular permeability.

Figure 6

Immunohistochemical staining of sectioned mouse brains revealed the presence of γH2A.X within tumor tissue, indicating α-particle induced tumor cell killing 2 days (n=3) (A) and 5 days (n=3) (B) post intracranial infusion (p.i.) of 225Ac-IA-TLs. (C) Immunohistochemical staining of sectioned mouse brains also revealed negligible presence of γH2A.X in normal brain tissue surrounding tumors (0.5mm from GBM border) (n=3), indicating absence of double strand (ds) DNA breaks within these regions where enhancement in vascular permeability (extravasation of Evans Blue dye) was observed. Data represented as mean +/− SEM. Student’s t-test and One-way ANOVA was performed to assess difference between experimental groups (*p< 0.001(significant)).