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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2017 Jun 27;16(10):2120–2129. doi: 10.1158/1535-7163.MCT-17-0176

Figure 2.

Figure 2

The effect of EPPI and CPPI on R1881-induced GFP-AR nuclear localization. a. HEK293 cells were transiently transfected with GFP-AR (green) in androgen-free conditions. The transfected cells were treated with vehicle DMSO, EPPI or CPPI at 25 μM in the presence of indicated concentrations of R1881 overnight. The subcellular localization of GFP-AR was visualized using fluorescent microscopy. The nuclei were stained with DAPI (blue). b. PC-3 cells were transiently transfected with GFP-AR as in a. c. Quantification of cells exhibiting nuclear localization in transfected HEK293 (left panel) or PC-3 cells (right panel). Data are expressed as mean ±SD (*, p < 0.05).