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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2017 Jun 27;16(10):2120–2129. doi: 10.1158/1535-7163.MCT-17-0176

Figure 3.

Figure 3

Inhibition of AR transcriptional activation of PSA by EPPI or CPPI in C4-2 cells. a. C4-2 cells cultured in complete media were treated with 25 μM EPPI or CPPI for 48 and immunoblotted with AR and PSA antibodies. GAPDH served as internal loading control. b. The effect of EPPI on PSA promoter-driven luciferase activity in C4-2 cells. The indicated concentrations of EPPI were added to C4-2 cells pre-cultured in androgen-free media and transiently transfected with PSA6.1-luciferase and tk-renilla luciferase reporters in the presence of 0.1 nM R1881 or vehicle ethanol for 48 h. c. The effect of CPPI on PSA promoter-driven luciferase activity in C4-2 cells as described in b. Firefly luciferase activity was normalized to tk promoter-driven renilla luciferase activity. Data are expressed as mean ±SEM (***, p < 0.001).