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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Exp Eye Res. 2017 Aug 10;164:118–128. doi: 10.1016/j.exer.2017.08.007

Fig. 4. Relative cytokine levels in alkali burned corneas and the effect of SLURP1 on TNF-α-induced cytokine expression in HUVEC.

Fig. 4

A. Relative levels of transcripts encoding Ifna, Ifnb, Tnfa, Cxcl1 and Cxcr2 in alkali-burned mouse corneas on post-burn days-2 and -7. RNA was extracted from control contralateral and alkali-burned mouse corneas on post-burn days-2 and -7, and the transcript levels tested by QPCR. B. Compound panel chart showing the effect of SLURP1 on expression of different cytokine transcripts in HUVEC cells. RNA was extracted from HUVEC cells pretreated with SLURP1 or control protein and exposed to TNF-α for 4 h and transcript levels measured by QPCR. Y-axis is presented as a compound panel, with the scale shifting to multiples of 10 after 5, to accommodate the large increase in IL-8 expression in HUVEC cells following TNF-α treatment. Results shown are the average of three independent experiments.