Figure 2.
Cisplatin selection enriches the API5high cell population of human cancer cells. (a) The diagram depicting the process of in vivo cisplatin selection among human cervical cancer cells. (b–d) Each experiment was performed in cancer cells at each stage with cisplatin-selected (P0, P1, P2 and P3 populations) and naïve cells (N1, N2 and N3 populations). (b) Flow cytometry analysis of the percentage of apoptotic (active caspase-3+) cells after treatment with 4 μM cisplatin for 36 h. (c) Western blot analysis of API5 expression. β-actin was included as an internal loading control. The numbers below the blot images indicate the expression as measured by fold change. Top, Quantification of API5 expression. Bottom, representative western blot images. (d) Flow cytometry analysis of cells expressing high levels of API5. Top, Representative FACS images. Bottom, Quantification of the frequency of cells with high levels of API5 expression. All the graphs represent three independent experiments performed in triplicate. Error bars represent the s.d.'s from the mean. *P<0.01, **P<0.005, ***P<0.001.