Figure 1.

Design of mouse experimental model. (a) After being fed MCDE for 2 weeks, mice were treated with MCDE for 2 additional weeks in parallel with i.p. injection of saline (M+V, n=6) or 50 ng of recombinant TSG-6 (M+TSG-6, n=6) every other day for 2 weeks. Chow-fed mice were injected with equal volume of vehicle (CON, n=4). (b) Mice fed MCDE for 2 weeks were additionally treated with MCDE for 2 weeks in parallel with everyday i.p. injections of 40 mg kg⁻¹ of 3-MA with (M+TSG-6+3-MA, n=5) or without TSG-6 (M+3-MA, n=4) for 2 weeks. As a CON, chow-fed mice were injected with equal volumes of vehicle (CON) or 3-MA (CON+3-MA, n=4) for 2 weeks.