Skip to main content
. 2017 Sep 22;49(9):e380. doi: 10.1038/emm.2017.140

Figure 3.

Figure 3

TSG-6 increases expression of autophagy markers in livers of MCDE-fed mice. (a) qRT-PCR analysis of atg3, atg7, lc3 and lamp2a in livers from CON, M+V, and M+TSG-6-treated mice results are plotted (mean±s.d. n⩾4 mice per group). (b) Western blot analysis and (c) cumulative densitometry analyses for LC3 (LC3-II: 14 kDa, processed form) (inducer of autophagosomes), LAMP2A (lysosome membrane protein) and RAB7 (autophagosome-lysosome docking marker) in livers of three representative mice from each group. GAPDH was used as an internal CON. Data shown represent one of three experiments with similar results. The results are displayed as the mean±s.d. (n⩾4 mice per group) (*P<0.05). (d) IHC for LC3-II in liver sections from representative chow- or MCDE-fed mice treated with vehicle or TSG-6 (X40). (e) Immunofluorescent staining for RAB7 in liver sections from each group. Representative images are shown (× 100, Scale bar: 10 μm). (f) Quantitative RAB7 immunofluorescent staining data from each group (n⩾4 mice per group). The number of RAB7-positive cells was counted in 10 fields per section. RAB7-positive cells were quantified by counting the total number of RAB7-positive cells with ⩾5 μm-sized DAPI-stained nuclei per field and dividing by the total number of cells with ⩾5 μm-sized DAPI-stained nuclei. Mean±s.d. results are plotted (*P<0.05).