Table 2.
Cultural characteristics and identification of the bacterial strains from Sophora tomentosa nodules.
| Strain | Cultural group | CCa | BPb | Most similar sequence found in the genbank |
||
|---|---|---|---|---|---|---|
| Accesion number | % of similarity | Species | ||||
| RIOP231 | 3 | RAC | 1253C | AJ420773 | 100 | Sinorhizobium/Ensifer adhaerens |
| RIOP243II | 3 | RAC | 774F | KU877657 | 100 | Sinorhizobium/Ensifer adhaerens |
| RIOP243III | 3 | RAC | 1248C | AJ420773 | 100 | Sinorhizobium/Ensifer adhaerens |
| RIOP243V | 3 | RN | 1002F | AJ420773 | 100 | Sinorhizobium/Ensifer adhaerens |
| RIOP245I1 | 3 | RAC | 1246C | AJ420773 | 100 | Sinorhizobium/Ensifer adhaerens |
| RIOP245I22 | 3 | RAC | 1287C | AJ420773 | 100 | Sinorhizobium/Ensifer adhaerens |
| RIOP245II1 | 3 | RAC | 1124C | AJ420773 | 100 | Sinorhizobium/Ensifer adhaerens |
| RIOP245II22 | 3 | RN | 1203C | AJ420773 | 100 | Sinorhizobium/Ensifer adhaerens |
| RIOP245III | 1 | RAC | 1226C | AJ420773 | 100 | Sinorhizobium/Ensifer adhaerens |
| SIIIP3N1 | 1 | IAC | 1203C | NR115768 | 99 | Sinorhizobium mexicanus |
| NR116265 | 99 | Sinorhizobium chiapanecum | ||||
| RIOP321 | 3 | RAC | 1267C | KT380607 | 100 | Rhizobium pusense |
| RIOP322 | 3 | RAC | 1276C | KT380607 | 100 | Rhizobium pusense |
| RIOP323 | 3 | RAC | 1244C | KT380607 | 100 | Rhizobium pusense |
| RIOP324 | 3 | RAC | 1256C | KT380607 | 100 | Rhizobium pusense |
| RIOP325 | 3 | RAC | 1254C | KT380607 | 100 | Rhizobium pusense |
| RIOP243IV | 4 | RAL | 1220C | KT580622 | 100 | Brevibacillus brevis |
| KT719605 | 100 | Brevibacillus formosus | ||||
| SIIP1N1I | 4 | RAL | 1297C | NR112926 | 99 | Brevibacillus nitrificans |
| NR040980 | 99 | Brevibacillus choshinensis | ||||
| RIOP344 | 2 | RAC | 1401C | GU985441 | 99 | Paenibacillus kribbensis |
| RIOP251I1 | 2 | RAC | 1310C | KY407752 | 100 | Bacillus aryabhattai |
| RIOP251II | 5 | RAC | 1405C | KY407752 | 100 | Bacillus aryabhattai |
| RIOP346 | 5 | RN | 1306C | KU156696 | 100 | Bacillus cereus |
| KY003095 | 100 | Bacillus thuringiensis | ||||
a
Cultural characteristics growth and change of pH: RAC, rapid growth and acidic pH; RAL, rapid growth and alkaline pH; RN, rapid growth and neutral pH; IAC, intermediate growth and acid pH.
b
Number of base pairs in the 16S RNA sequence used for identification: F, foward; C, Contig. Strains with prefix “RIO” were isolated from nodules collected in the field and the prefix “SI” from the nodules collected in trap plants cultivated in axenic condition and inoculated with soil from the rhizosphere.