Table 1.
Purification of C12O enzyme from PA (Corynebacterium aurimucosum), PM (Acinetobacter baumannii) and BM (Microbacterium hydrocarbonoxydans).
| Bacterial strains | PA |
PM |
BM |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Purification steps | Bacterial cell lysate | (NH4)2SO4 precipitation (60%) | DEAE cellulose (0.3 M NaCl) | Bacterial cell lysate | (NH4)2SO4 precipitation (60%) | DEAE cellulose (0.3 M NaCl) | Bacterial cell lysate | (NH4)2SO4 precipitation (60%) | DEAE cellulose (0.3 M NaCl) |
| Volume (mL) | 10 | 5 | 3 | 10 | 5 | 3 | 10 | 5 | 3 |
| Protein (mg/mL) | 2.84 | 1.11 | 0.86 | 1.35 | 0.98 | 0.42 | 3.58 | 1.64 | 0.98 |
| Activity (U) | 28.12 | 21.7 | 14.56 | 4.12 | 3.25 | 2.32 | 65.87 | 30.01 | 18.55 |
| Specific activity (U mg−1) | 0.99 | 3.91 | 5.64 | 0.31 | 0.66 | 1.84 | 1.84 | 3.66 | 6.37 |
| Purification fold | 1 | 3.94 | 5.7 | 1 | 2.17 | 6.03 | 1 | 1.98 | 3.73 |
| Recovery (%) | 100 | 38.59 | 15.52 | 100 | 39.44 | 16.89 | 100 | 22.45 | 8.44 |