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Construction of recombinant plasmid pGPA. After cloning using T vector, an intact plasmid including the promoter Pglv was inserted into plasmid pGJ103 (3.2 kb), resulting in the construction of pGP (3.45 kb). The plasmid containing the genes of abaecin and TEV protein, with gmp and gsp, was digested with EcoRI and BamHI, while pGP was digested with EcoRI and BamHI. The two digested products were linked by T4 DNA ligase, yielding recombinant plasmid pGPA.
