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. 2017 Jun 3;48(4):809–814. doi: 10.1016/j.bjm.2017.01.009

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© 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Expression of abaecin in Bacillus subtilis 1A747. The analysis of purified abaecin on Tricine-SDS-PAGE and Western Blot revealed its molecular mass to be about 4 kDa. (a) SDS-PAGE analysis of abaecin expressed. Lane 1, total cell protein (uninduced); lane 2, total cell protein (induced); lane M, low-range molecular mass marker (TaKaRa, Shiga, Japan). (b) Western blot analysis of abaecin expressed. Lane 3, total cell protein (uninduced); lane 4, total cell protein (induced). The arrow indicates the position of recombinant abaecin.