Fig. 4.

Effects of KGE on zebrafish embryos SIV length. Zebrafish embryos at 24 h.p.f. were incubated with KGE (500 or 1000 µg/ml) for 48 h. The embryos were stained and images were captured under microscope. The upper panel indicated the quantification of the length of SIV by Image J software, the lower panel showed the SIV of the zebrafish. Values are presented as the means ± SD. At least 30 embryos were assessed in each condition. All data were analysed by ANOVA with multiple post hoc testing. * p < 0.05 vs control.