Fig. 5. In vitro cellular toxicity of BKM120-loaded HE12–SNAs. Cytotoxicity of BKM120-loaded nanoparticles when administered at concentrations (a) 0.12 μM (b) 1.2 μM (c) 12 μM prior to doxorubicin treatment, measured over 24 hours. *** corresponds to p < 0.0001 and ** to p < 0.001. Error bars represent the standard deviation of measurements. (d) Annexin/PI staining and (e) cleaved caspase-3 assay showing the potency of BKM120-loaded particles at inducing apoptosis in primary patient B-CLL lymphocytes in the presence of the BMS2 stromal cells support (cocultured CLL), analyzed by flow cytometry.
