Fig. 2.

Rg1 enhances the heterodimerization of E protein with MyoD. (A) 293T cells transfected with MyoD were treated with Rg1 for 36 hrs, followed by immunoprecipitation with anti-E2A antibodies and immunoblotting using anti-MyoD antibodies. Cell lysates are used as an input control for each protein. (B) C2C12 myoblasts were pretreated with 1μM Akt inhibitor (LY294002) or 2.5μM p38MAPK inhibitor (SB203580). After 30 min, C2C12 myoblasts were treated with Rg1 and then differentiated for 48 hrs. Total lysates were followed by immunoprecipitation with anti-E2A antibodies and immunoblotting analysis using anti-MyoD antibodies. Total lysates are used as the input control.