Skip to main content
. 2017 Jun 7;41(4):608–614. doi: 10.1016/j.jgr.2017.05.006

Fig. 5.

Fig. 5

Rg1 augments myogenic differentiation of 10T1/2 embryonic fibroblasts transfected with MyoD. (A) MyoD-transfected 10T1/2 fibroblasts were treated with either vehicle or 10nM Rg1 and then differentiated in DM for 2 d. Muscle-specific proteins were analyzed by immunoblotting. Pan-Cadherin was used as the loading control. (B) The signal intensity of indicated muscle-specific proteins was quantified in three independent experiments and normalized to the loading control pan-Cadherin. The values from DMSO-treated MyoD-transfected cells were set to 1.0. Values are presented as means ± SD. (C) The total and phosphorylated forms of p38MAPK and Akt of cell lysates from panel (A) were analyzed by immunoblotting. (D) The signal intensity of total and phosphorylated forms of p38MAP and Akt was quantified in three independent experiments, and the relative values for the phosphorylated forms to total p38MAPK and Akt proteins were determined, respectively. The values from DMSO-treated MyoD-transfected cells were set to 1.0. Values are presented as means ± SD. * p < 0.05 and ** p < 0.01 compared to control group. DMSO, dimethyl sulfoxide; MHC, myosin heavy chain; NS, not significant; SD, standard deviation.