Table 2.

Validation of the method for the LC–MS/MS determination of ginsenosides. LOD, limit of detection; LOQ, limit of quantitation; RSD, relative standard deviation

Ginsenosides	Calibration curve equation	Correlation coefficient	Range (ng/mL)	LOD (ng/mL)	LOQ (ng/mL)	Recovery % (RSD%)
						100 ng/mL	1,000 ng/mL	2,000 ng/mL
Re	y = 10.691x+ 2519.7	R2 = 0.9973	50–5,000	7.51	24.99	104.4 (8.8)	94.8 (6.6)	116.5 (7.4)
Rg1	y = 13.074x + 7896.7	R2 = 0.9930	50–5,000	4.52	15.06	104.2 (11.1)	116.8 (10.1)	104.2 (8.9)
Rf	y = 9.7808x + 4535.2	R2 = 0.9940	50–5,000	4.23	14.09	98.9 (8.9)	98.2 (4.8)	109.2 (9.4)
Rb1	y = 4.7875x + 360.18	R2 = 0.9964	50–5,000	12.01	40.03	97.6 (10.0)	101.9 (0.6)	106.2 (2.6)
Rc	y = 1.9243x + 520.16	R2 = 0.9951	50–5,000	15.05	50.17	95.2 (7.3)	95.1 (9.1)	100.4 (4.5)
Rb2	y = 1.6348x + 545.69	R2 = 0.9955	50–5,000	12.04	40.12	90.4 (5.0)	80.1 (8.8)	109.1 (8.7)
Rd	y = 10.886x + 1745.9	R2 = 0.9917	50–5,000	2.27	7.56	104.9 (6.9)	101.7 (4.1)	95.5 (3.9)
Rg2	y = 0.5688x + 389.94	R2 = 0.9902	50–5,000	20.05	66.83	115.8 (9.6)	94.5 (10.7)	111.7 (7.7)
Rh1	y = 2.6693x + 170.07	R2 = 0.9987	50–5,000	3.33	11.11	123.7 (9.5)	107.7 (8.1)	107.2 (2.2)
F1	y = 4.189 x + 1495.3	R2 = 0.9905	50–5,000	9.38	31.25	96.1 (8.1)	100.5 (0.4)	95.9 (4.9)
F2	y = 11.384x + 4093.2	R2 = 0.9910	50–5,000	2.64	8.79	104.9 (6.5)	109.5 (11.5)	107.2 (2.3)
Rg3	y = 11.374x + 3971.8	R2 = 0.9916	50–5,000	13.63	45.43	112.0 (9.2)	99.2 (10.1)	105.2 (7.6)
PPT	y = 5.7564x + 1116.6	R2 = 0.9971	50–5,000	7.23	24.09	109.3 (4.8)	86.5 (7.6)	102.9 (0.9)
Compound K	y = 6.5315x + 1551.7	R2 = 0.9953	50–5,000	5.35	17.83	78.5 (8.2)	101.4 (9.6)	82.5 (8.8)
Rh2	y = 5.5618x + 1891.3	R2 = 0.9978	50–5,000	4.68	13.59	82.0 (9.2)	99.5 (8.7)	113.7 (9.1)