Table 4.
Accuracy of UPLC method for the determination of reference compounds
| Analyte | Original (mg) |
Spiked (mg) |
Found (mg) |
Recovery (%) |
RSD (%) |
|---|---|---|---|---|---|
| Rg1 | 1.09 | 0.88 | 1.96 | 98.99 | 2.80 |
| 1.1 | 2.24 | 105.37 | 0.36 | ||
| 1.32 | 2.34 | 94.80 | 2.94 | ||
| Re | 4.19 | 1.2 | 5.48 | 106.83 | 2.85 |
| 1.5 | 5.69 | 99.53 | 0.85 | ||
| 1.8 | 6.01 | 101.05 | 2.62 | ||
| Rb1 | 0.58 | 0.4 | 0.98 | 98.94 | 4.52 |
| 0.5 | 1.04 | 92.82 | 1.24 | ||
| 0.6 | 1.19 | 101.99 | 0.42 | ||
| Rc | 1.02 | 0.64 | 1.68 | 104.44 | 0.96 |
| 0.8 | 1.82 | 100.13 | 0.33 | ||
| 1.06 | 1.98 | 90.64 | 1.74 | ||
| Rb2 | 0.70 | 0.44 | 1.15 | 100.80 | 0.90 |
| 0.55 | 1.24 | 97.98 | 0.57 | ||
| 0.66 | 1.34 | 97.27 | 1.24 | ||
| Rb3 | 0.20 | 0.16 | 0.33 | 84.17 | 6.46 |
| 0.20 | 0.36 | 82.42 | 1.26 | ||
| 0.24 | 0.42 | 91.73 | 2.84 | ||
| Rd | 2 | 1.60 | 3.66 | 104.05 | 1.15 |
| 2.00 | 3.95 | 97.18 | 0.62 | ||
| 2.40 | 4.32 | 96.84 | 1.49 |
RSD, relative standard deviation; UPLC, . ultraperformance liquid chromatography