Fig. 8. The images of living HeLa cells pre-treated with HMN (5 μM) for 30 min and subsequently co-incubated with (A) Mito-Tracker Green (1 μM) or (B) Lyso-Tracker Green (1 μM) for 10 min. (A1, B1) Green channel images of Mito-Tracker Green and Lyso-Tracker Green (λ ex = 488 nm, λ em = 500–535 nm); (A2, B2) red channel images of probe HMN (λ ex = 561 nm, λ em = 595–630 nm); (A3, B3) merged green and red channel images; (A4, B4) the overlap coefficients of green and red channel images; (A5, B5) the intensity profile of the ROI in merged images. Scale bar: 10 μm.
