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. 2017 Oct 4;18:44. doi: 10.1186/s12865-017-0227-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Representative flow cytometry plots. Dot plot of forward-angle versus right-angle light-scattering properties; the oval marks indicate the electronic windows used for analysis of fluorescence data for lymphocyte precursors; the rectangular boxes indicate the electronic windows used for analysis of fluorescence data for monocyte precursors with percentages. a A quadrant has been set to delineate the CD19 and IL-6 cells. Murine splenocytes were stimulated with water + water, water + CpG-B, iSN34 + water, or iSN34 + CpG-B for 12 h, and then sorted into CD19⁺ IL-6⁺ cells. b Mean percentage of IL-6⁺ CD19⁺ cells in the total population was determined in each group. Similar results were obtained from at least three different mice. Values are presented as mean + SD of three independent experiments, each performed in triplicate (n = 9). Values with different letters (i.e., a, b, c, and d) were significantly different (p < 0.01)