Fig. 5.

MiR-216b suppresses HeLa cell proliferation by inhibiting FOXM1. a The expression levels of FOXM1 (upper left) and miR-216b (bottom left) in cervical cancer tissues of 8 patients were determined by western blotting and real-time RT-PCR, respectively. The relative band intensity values of FOXM1 normalized by β-actin were shown above the β-actin bands. The levels of miR-216b and FOXM1 in cervical cancer tissues were negatively correlated. Correlation analysis (right) confirmed the negative correlation between miR-216b and FOXM1. b FOXM1 expression was suppressed by FOXM1-siRNA in both negative control HeLa cells (NC) and miR-216b inhibitors transfected cells (miR-216b-in). Normalized FOXM1 expression was shown. HeLa cells were transfected with or without FOXM1-siRNA and miR-216b inhibitors and measured by western blotting analysis. β-actin served as an internal control. * P < 0.05. c MTT assay of cell proliferation in FOXM1-silenced HeLa cells. FOXM1-siRNA transfection suppressed the proliferation of HeLa cells, whereas in miR-216b inhibitors transfected cells, the enhanced cell proliferation could be repressed by FOXM1 silencing