Fig. 3.

Receptor-interacting serine-threonine kinase 3 (RIPK3) signaling contributes to the aberrant response to K/BxN serum-transfer-induced arthritis in Cre ^LysM Casp8 ^flox/flox and Cre ^CD11c Casp8 ^flox/flox mice. a-d Male 10 − 12-week-old Casp8 ^flox/flox (control, n = 17), Cre ^LysM Casp8 ^flox/flox (n = 28) and RIPK3 ^–/– Cre ^LysM Casp8 ^flox/flox (n = 33) mice were intravenously injected with K/BxN serum. a Depicted are combined “change in ankle width” and “clinical score” from three individual experiments. Differences between Cre ^LysM Casp8 ^flox/flox and RIPK3 ^–/– Cre ^LysM Casp8 ^flox/flox mice were tested by two-way analysis of variance with the Bonferroni post-hoc test: *p < 0.05; **p < 0.005; ***p < 0.0005. b Day-11 ankles from control (n = 3), Cre ^LysM Casp8 ^flox/flox (n = 5) and RIPK3 ^–/– Cre ^LysM Casp8 ^flox/flox (n = 5) mice stained with hematoxylin and eosin (H&E). P = pannus; SL = synovial lining; C = cartilage; B = bone; BM = bone marrow. c, d Histologic scoring of day-11 H&E-stained ankle sections. Data are means ± SEM and are compared between control and Cre ^LysM Casp8 ^flox/flox mice by the Mann-Whitney test: ^# p < 0.05. e-h Male 10–12-week-old control (n = 20), Cre ^CD11c Casp8 ^flox/flox (n = 26) and RIPK3 ^–/– Cre ^CD11c Casp8 ^flox/flox (n = 21) mice were intravenously injected with K/BxN serum. e Depicted are combined “change in ankle width” and “clinical score” from three individual experiments. Differences between Cre ^CD11c Casp8 ^flox/flox and RIPK3 ^–/– Cre ^CD11c Casp8 ^flox/flox mice were compared by two-way analysis of variance with the Bonferroni post-hoc test: *p < 0.05; ***p < 0.0005. f Day-3 ankles stained with H&E. g, h Histologic scoring of day-3 H&E-stained ankle sections from from control (n = 3), Cre ^CD11c Casp8 ^flox/flox (n = 5) and RIPK3 ^–/– Cre ^CD11c Casp8 ^flox/flox (n = 5) mice. Data are means ± SEM and were compared between control and Cre ^CD11c Casp8 ^flox/flox mice by the Mann-Whitney test: ^# p < 0.05 and between Cre ^CD11c Casp8 ^flox/flox and RIPK3 ^–/– Cre ^CD11c Casp8 ^flox/flox mice by the Mann-Whitney test: **p < 0.005; ***p < 0.0005