Fig. 7. Competition analysis for 36 with the acetylated lysine substrate. (A) Michaelis–Menten plot showing the Fluor de Lys SIRT2 substrate (μM) competition analysis for of 36 at 0, 0.02, 0.06 and 0.2 μM. (B) Lineweaver–Burk plot: 1/V as a function of the reciprocal acetylated lysine substrate in the presence of 0, 0.02 and 0.06 μM of 36. Values were calculated from two independent determinations.
