Fig. 3. Virtual time-lapse FDAA labeling. (A) Streptomyces venezuelae cells were sequentially labeled with Atto₄₈₈ADA (3 h), Cy_3BADA (15 min), AF₃₅₀DL (15 min), and Atto₆₁₀ADA (15 min), and then fixed and imaged. Left: Phase-contrast; right: fluorescence. Scale bar: 5 μm. White arrows point out new branches formed at different time points (oldest to newest: (a) to (c)). (B) Lactococcus lactis cells were sequentially labeled with Atto₆₁₀ADA (8 min), HADA (5 min), YADA (8 min), sBADA (5 min), and TADA (5 min), and then fixed and imaged. Left to right: Phase channel, Atto₆₁₀ADA, HADA, YADA, sBADA, TADA, and merged image. Scale bar: 1 μm.