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. 2017 Oct 3;(128):56101. doi: 10.3791/56101
Table 1: Examples of Markers for the C. elegans Excretory Canal Membrane System
Protein name  Subcellular localization Function Examples of available strains References
ERM-1 apical domain membrane–cytoskeleton linker VJ402 (fgEx13[erm-1p::erm-1::gfp, rol-6p::rol-6(su1006)]) ref (13)
ACT-5 apical domain apically localized actin VJ268 (fgEx12[(act-5p::act-5::gfp); unc-119(+);unc-119(ed3)III]) ref (13)
ABTS-2 Basolateral Anion/bicarbonate transporter ABTS-2::GFP ref (42)
SULP-8 Basolateral Sulfatepermease SULP-8::GFP ref (42)
VHA-1 canalicular vesicles V-ATPase VJ535 (fgEx35(vha-1p::vha-1::gfp; rol-6p::rol-6(su1006))) ref (9)
VHA-5 canalicular vesicles V-ATPase ML846 (vha-5(mc38) IV;mcEx337[vha-5(+)::GFP + rol-6(su1006)] ) ref (10)
AQP-8 canalicular vesicles aquaporin, water channel VJ533 (fgEx33(aqp-8p::aqp-8::gfp)) ref (9)
RAB-5 endosomal vesicles trafficking BK209 (qpIs99 (exc9p::mCherry::rab-5)) ref (36)
RAB-7 endosomal vesicles trafficking BK210 (qpIs100 [Pexc-9::mCherry::rab-7]) ref (36)
RAB-11 endosomal vesicles trafficking BK205 (qpIs97 (exc9p::mCherry::rab-11) ) ref (36)
1Examples are selected from resources listed in Table 3.