Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 27;16(19):1781–1789. doi: 10.1080/15384101.2017.1281483

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Taylor & Francis

PMC Copyright notice

Figure 2. — Expression of double mutants containing the H3.3K36M and each Asf1 binding deficient mutant results in reduced H3K36me2/me3 levels in T/C28a2 cells. T/C28a2 cells were infected with virus expressing FLAG-tagged wild type H3.3, or H3.3 mutants with relevant mutations indicated in the figure (A-D). Cells were collected for chromatin fractionation assays to separate proteins into soluble and chromatin fractions. Proteins in whole cell extracts (WCE), chromatin and soluble fractions were analyzed by Western blot.