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Binding between bacteria and rTbgTCTP as revealed by western blot using anti-His mAb. Lane 1: molecular weight markers (Novex Sharp Pre-Stained Protein Standard, Thermo Fisher Scientific); lane 2: purified rTbgTCTP; lane 3: bacteria (Providencia spp. from Glossina palpalis palpalis) incubated without rTbgTCTP as a control; lane 4: bacteria incubated with rTbgTCTP and subsequently treated with β-mercaptoethanol.
