Figure 1.

Acid adaptation changes the phenotype of cancer cells toward more aggressive one: (A) size and shape of NA and AA MCF-7 cells. (B) Wound healing assay shows acid adapted MCF_7 cells are more migratory than non-adapted cells. (C) Acid adapted MCF-7 cells are more amenable to spheroiogenic. (D) Migration assay using Xcelligene system confirms the migratory behavior of acid adapted MDA-mb-231 (red) cancer cells compared to non-adapted (green) one in low pH media (P < 0.05 from 3 hours on).