Figure 2.

Acid adapted cells grow more rapidly in mouse xenografts. (A) Tumor size measured by caliper over 2 weeks (top) as well as statistical analysis of tumor size for day 14 (bottom) and (B) representative images of tumor bioluminescence after 14 d. The same number of acid adapted and non-adapted MDA-mb-231 cells were injected to nude mice. Acid adapted cells grew faster and make bigger tumors in vivo. (C) co-culture of acid adapted (red) and non-adapted (green) MCF-7 cells in a 3D spheroid model in acidic media (pH6.5). Acid adapted cells move to the edge of spheres, and may result in protecting the whole system from toxicity of acidic media.