Figure 1.

Estimates of the cultured fraction of human and mouse gut bacteria and archaea as detected by high-throughput sequencing of 16S rRNA amplicons. The analysis was based on 14,734 full-length sequences of isolates and 26,036 complex gut microbial profiles (16,667 from human and 9,369 from mouse). Single OTUs from these profiles were matched to the isolate sequences, and those with similarities above the conservative species- (97%) and genus- (95%) specific levels were considered to originate from cultured bacteria. Results were expressed either as percentage of OTU richness or corresponding cumulative relative abundance of sequences. The figure illustrates data obtained when considering OTUs that occurred at a relative abundance > 0.5%. For estimates based on all OTUs, readers are referred to Supplemental Figure S1.