Table S1.

Association of the frequency of methylation occurrence of cg20907471 (PAX1) and average of cg22303211 and cg06675478 (SOX1) in cancer tissues to the clinical characteristics of 166 patients with CRC using TCGA datasets (n=166)

Characteristics	N	SOX1				PAX1
		M	U	Methylated percentage	P-value	M	U	Methylated percentage	P-value*
Age, years					0.239				0.560
Mean (SD) [range]	70	(11.9)	(36–90)
<50	15	7	8	46.67		3	12	20.00
50–70	61	42	19	68.85		21	40	34.43
>70	90	54	36	60.00		29	61	32.22
Gender					0.631				0.617
Male	83	53	30	63.86		28	55	33.73
Female	83	50	33	60.24		25	58	30.12
M					0.576				0.191
M0	142	88	54	61.97		43	99	30.28
M1	24	15	9	62.50		19	14	79.17
N					0.329				0.209
N0	99	65	34	65.66		27	72	27.27
N1	32	20	12	62.50		14	18	43.75
N2	34	18	17	52.94		12	23	35.29
T					0.310				0.834
T1	4	1	3	25.00		2	2	50.00
T2	35	24	11	68.57		10	25	28.57
T3	110	69	41	62.73		36	74	32.73
T4	17	9	8	52.94		5	12	29.41
Stage					0.666				0.646
I	32	22	10	68.75		9	23	28.13
II	64	41	23	64.06		18	46	28.13
III	45	25	20	55.56		16	29	35.56
IV	24	15	10	62.50		10	15	41.67
CEA					0.338				0.887
<9.7	78	46	32	58.97		26	52	33.33
>9.7	24	13	11	54.17		8	16	33.33
NA	64	44	20	68.75		19	45	29.69

Note:

^*Calculated χ² test.

Abbreviations: CEA, carcinoembryonic antigen; CRC, colorectal cancer; M, methylated; PAX1, paired boxed gene 1; SOX1, sex-determining region Y-related high-mobility group box 1; TCGA, The Cancer Genome Atlas; U, unmethylated.