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. 2017 Sep 28;10:4753–4763. doi: 10.2147/OTT.S143787

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© 2017 Wang et al. This work is published and licensed by Dove Medical Press Limited

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miR-147a inhibition abrogates the effect of HOXD-AS1 knockdown on NSCLC cell proliferation by regulating pRB expression.

Notes: A549 cells were transfected with si-NC, si-HOXD-AS1, si-HOXD-AS1+miR-147a inhibitor, and miR-147a inhibitor. (A) The expression of miR-147a in these cells. (B) CCK-8 assay was performed to examine cell proliferation. (C) Cell cycle status was determined. (D) Cell apoptosis was detected by flow cytometry. (E) HOXD-AS1 positively correlated with pRB expression in NSCLC (GDS3627). mRNA (F) and protein (G) levels of pRB were examined in cells transfected with si-NC, si-HOXD-AS1, and si-HOXD-AS1+miR-147a inhibitor. *P<0.05; **P<0.01; ***P<0.001.

Abbreviations: CCK-8, cell counting kit-8; HOXD-AS1, HOXD cluster antisense RNA 1; NC, negative control; NSCLC, non-small cell lung cancer; pRB, retinoblastoma protein; FL, fluorescence; PI, propidium iodide.

miR-147a inhibition abrogates the effect of HOXD-AS1 knockdown on NSCLC cell proliferation by regulating pRB expression.

Notes: A549 cells were transfected with si-NC, si-HOXD-AS1, si-HOXD-AS1+miR-147a inhibitor, and miR-147a inhibitor. (A) The expression of miR-147a in these cells. (B) CCK-8 assay was performed to examine cell proliferation. (C) Cell cycle status was determined. (D) Cell apoptosis was detected by flow cytometry. (E) HOXD-AS1 positively correlated with pRB expression in NSCLC (GDS3627). mRNA (F) and protein (G) levels of pRB were examined in cells transfected with si-NC, si-HOXD-AS1, and si-HOXD-AS1+miR-147a inhibitor. *P<0.05; **P<0.01; ***P<0.001.

Abbreviations: CCK-8, cell counting kit-8; HOXD-AS1, HOXD cluster antisense RNA 1; NC, negative control; NSCLC, non-small cell lung cancer; pRB, retinoblastoma protein; FL, fluorescence; PI, propidium iodide.