Skip to main content
. Author manuscript; available in PMC: 2017 Oct 5.
Published in final edited form as: Small. 2015 Jul 20;11(38):5066–5078. doi: 10.1002/smll.201500937

Figure 5.

Figure 5

Killing of M. tuberculosis in human macrophages by free INH and the 100 nm and 50 nm INH-loaded pH-responsive nanoparticles coated with PEI-PEG. THP-1 macrophages were infected with M. tuberculosis and treated with various concentrations of A) MSN-CHO-INH-PEI-PEG, B) SMSN-CHO-INH-PEI-PEG or C) INH. The killing effect of supernates prepared from D) MSN-CHO-INH-PEI-PEG and E) SMSN-CHO-INH-PEI-PEG under neutral or acidic conditions on intracellular M. tuberculosis were compared with the killing effect of no treatment or treatment with INH-loaded pH-responsive nanoparticles. F) Median-effect plot, where D is the dose of INH; Fa/Fu is the Fraction of bacteria killed/Fraction of bacteria surviving. Data are represented as means ± standard errors (some are smaller than the symbol) with two biological and two technical repeats.