Figure 2.

Ex Vivo Studies of rhMG53 Efficacy on Single FDB Fibers Derived from Dysf^–/– Mice

(A) Representative images. (B and C) Time-dependent accumulation of FM4-64 dye (B) and the area under the curve (AUC) (C) demonstrate that rhMG53 protein efficiently accelerates membrane resealing in the micromolar concentration range on individual fibers damaged by an infrared laser. (D) The maximum fluorescence intensity indicates the mean peak value at 51 s seen for the FM4-64 signal in each group over the course of the experiment. White arrows indicate a wounding site at 0 and 45 s after laser injury. Scale bars represent 10 μm. Data are means ± SEM (n = 10–17). The Student’s t test was used for statistical analysis.