Figure 4.

Effect of rhMG53 Treatment on Serum Markers in Dysf^–/– Mice in Response to Eccentric Exercise

(A and B) Creatine kinase (CK) (A) and LDH (B) levels after eccentric exercise demonstrate rhMG53 efficacy as a protein drug to prevent muscle disruptions. CK and LDH levels in serum of sedentary C57BL/6J (WT) mice show the contribution of dysferlin deficiency on those cellular markers. Control mice (Contr) are considered to be sedentary Dysf^–/– (KO) mice. These were compared with exercised Dysf^–/– mouse serum samples. (C) Western blot of serum from Dysf^–/– mice shows nonspecific MG53 bands and i.p. delivered rhMG53 protein level in eccentric exercised rhMG53-treated group (top). The higher molecular weight MG53 oligomers (indicated by black arrows) were detected only in the rhMG53-treated group. The Ponceau S solution blot displays that similar amounts of serum proteins were loaded in the gel and then transferred to a PVDF membrane (bottom of C). Data are means ± SEM (n = 6 for A and B; n = 3 for C). The Benjamini, Krieger, and Yekutieli ANOVA test was used for statistical analysis.