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. 2017 Oct 5;12(10):e0185327. doi: 10.1371/journal.pone.0185327

Fig 9. FAIM-S_2a and FAIM-L_2a increase NGF induced neurite outgrowth in PC12 cells.

Fig 9

A: Cell death was measured in SK-N-AS cells by counting condensed nuclei after Hoechst 33258 (0.05 μg/ml) staining. Cells were transfected with empty vector (pcDNA3-FLAG) or pcDNA3-FLAG-containing all the isoforms and then treated or not with FasL (100 ng/ml). Graph represents the percentage of apoptotic nuclei of a minimum of 500 cells per condition. Experiments were repeated at least three times. Statistical significance was determined by one-way ANOVA. ns: no significant and *p<0.05;**p<0.01. B: Neurite length was measured in PC12 cells treated with NGF (100ng/ml) or left untreated after transfection of the isoform vectors. At least three independent experiments were performed. Statistical significance was determined by one-way ANOVA, ***p<0.001. C: Representative images of neurite outgrowth in the different conditions. UT: untreated; NGF: nerve growth factor (100 ng/ml) (n = 3 per experiment).