Table 1. List of specific primers for each exon used for RT-PCR.
Primer Name | Region Amplified | Direction | Sequence 5 -> 3 |
---|---|---|---|
1aF | Faim exon 1a | F | CTTCGGCTAAGGCAGAGGA |
1bF1 | Faim exon 1b | F | GACTACGTCGTGGGATCG |
1bF2 | Faim exon 1b | F | TGGTGAAACCTACCCCAGAG |
2aF | Faim exon 2a | F | TGGCCCATTCTATCCTATGC |
2bF | Faim exon 2b | F | GCATCTGGAGATGACAGTCC |
2aR | Faim exon 2a | R | GCAGAGTCCGGAGATACCAA |
2a3R | Faim 2a – 3junction | R | TAGGCTGTAAGGAGGGCTCA |
2bR | Faim exon 2b | R | CCATGGTTGGCAAAAACAGTCTCA |
3R | Faim exon 3 | R | TGCCTGATGTAGTCCCATGT |
6R | Faim exon 6 | R | TTCCGCTTCCCACTACTGAC |
nSR100F | NSR100 | F | ATTGTCGCCAGTATCACGGC |
nSR100R | NSR100 | R | TTTCTTGTGCCTCTTCTCATCTC |
L27F | RPL27 | F | AGCTGTCATCGTGAAGAA |
L27R | RPL27 | R | CTTGGCGATCTTCTTCTTGCC |
F: Forward; R: Reverse.