Fig 1. Growth inhibition of M. luminyensis by various AMPs.

A) 2x10⁷ cells of M. luminyensis incubated with depicted concentrations of LL32 at 37°C in 250 μl minimal medium under anaerobic conditions (see Materials and Methods). Turbidity of cultures at 600 nm (OD₆₀₀) was monitored over time. Error bars represent standard deviation of three biological replicates in one experimental setup. B) 2x10⁷ cells incubated with various concentrations of the peptides LL32, Lpep 19–2.5 or NK2 at 37°C in 250 μl minimal medium. Turbidity of control cultures at 600 nm (OD₆₀₀) after 200 h of growth was set to 100%. Error bars represent standard deviation of three biological replicates. C) Phase-contrast micrographs taken after 200 h of growth with the indicated concentrations of the respective added AMPs.