Figure 1.

Microfluidics Are an Enabling Platform for More Efficient LV Transduction

(A) Microfluidics bring LV particles within closer proximity to target cells without requiring large quantities that would otherwise be wasted due to their short half-life. (B) Example of microfluidic devices that accommodate 10⁶ (top) and 10⁵ (bottom) cells with a surface area equivalent to a 6-well plate and a 96-well plate, respectively. The scale bar represents 1 cm. (C) Transductions using the same amount of GFP-LV and Jurkat cells (constant MOI) for various volumes/fluid heights reveal a height range (<100 μm) in which diffusion no longer limits vector integration as indicated by the plateau (n = 2–4). Data represent means ± SD. (D) Jurkat transductions comparing utilization efficiencies for a constant GFP-LV (v/v)% concentration at various transduction volumes demonstrates that minimizing total volume more efficiently utilizes available LV (n = 2–4). Data represent means ± SD. (E) A constant ratio of cells and virus (MOI of 1) loaded into 10⁶ and 10⁷ scale microfluidics for 5 hr produces nearly identical transduction of Jurkat cells with a GFP-LV. Data represent mean ± SD. Insets are photos of different versions of microfluidics used for each condition. The scale bar represents 3 cm.