Skip to main content
. 2017 Jul 5;25(10):2394–2403. doi: 10.1016/j.ymthe.2017.06.028

Figure 2.

Figure 2

HOTAIR Promotes Trophoblast Invasion In Vitro and Trophoblast Outgrowth in Extravillous Explant Cultures In Vivo

(A and B) qRT-PCR analysis of HOTAIR expression in HTR-8 cells transfected with siCtrl, siHOTAIR-1, siHOTAIR-2, control vector, or HOTAIR-overexpressing vector after 48 hr. (C) HTR-8 cells were transfected with one of the above-mentioned vectors and siRNA. Cell proliferation was measured after at indicated time using the CCK-8 assay. *p < 0.05 compared with siCtrl or control vector. (D–F) HOTAIR overexpression in HTR-8 cells significantly increased cell invasion compared to the vector control cell line (top panels). HOTAIR knockdown reduced cell invasion compared to the scrambled control cell line (original magnification ×200). *p < 0.05 versus siCtrl or control vector. (G) Extravillous explants were obtained from healthy controls at 6–8 weeks of gestation and cultured on Matrigel. Serial pictures of the explants incubated with siHOTAIR or siCtrl were taken under a light microscope after 24 and 72 hr of in vitro culture (original magnification ×100). (H) Statistical assay of the migration distance of villous tips (%). Data are presented as means ± SD of three independent experiments.